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KMID : 0380220070400060973
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Journal of Biochemistry and Molecular Biology
2007 Volume.40 No. 6 p.973 ~ p.978
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SEPT12 Interacts with SEPT6 and This Interaction Alters the Filament Structure of SEPT6 in Hela Cells
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Ding Xiangming
Yu Wenbo
Liu Ming
Shen Suqin
Chen Fang
Wan Bo
Yu Long
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Abstract
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Septins are a family of conserved cytoskeletal GTPase forming heteropolymeric filamentous structure in interphase cells, however, the mechanism of assembly are largely unknown. Here we described the characterization of SEPT12, sharing closest homology to SEPT3 and SEPT9. It was revealed that subcelluar localization of SEPT12 varied at interphase and mitotic phase. While SEPT12 formed filamentous structures at interphase, it was localized to the central spindle and to midbody during anaphase and cytokinesis, respectively. In addition, we found that SEPT12 can interact with SEPT6 in vitro and in vivo, and this interaction was independent of the coiled coil domain of SEPT6. Further, co-expression of SEPT12 altered the filamentous structure of SEPT6 in Hela cells. Therefore, our result showed that the interaction between different septins may affect the septin filament structure.
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KEYWORD
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Co-localization, Filament structure, Protein interaction, Septin, Subcellular localization
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